I'm not sure I understand how you are close to retirement--are you counting the home equity? You only listed 345k of liquid that you can draw from, and that doesn't come close to supporting a 30k per year need. If your 30k per year expenses are 30k with a paid off house, then that 250k is getting you a return of whatever you would be paying rent. So you can't also count it for withdrawal rates. And to have a 4% withdrawal rate apply, the money has to be invested. You only have 160k currently invested, right? I'd work on getting the windfall into the market. Set up an automatic monthly investment and get it in slowly over six months, if that makes you feel more comfortable. But don't just sit on it.
On face value, however, if your expenses are only 30k, and you make 80k and your wife could still make 35k at an easier job, that's still more than enough for the two of you and a healthy savings rate. Since you mentioned having kids, have you looked into how that would increase your expenses? And if she is only working part-time, will your company's insurance cover her at a good rate? And does it have good maternity and family care? That's the stuff I'd be looking into if I was planning to have kids. Clearly many people take on new jobs just to get good insurance during this [potentially very expensive] time.
In my opinion, happiness and life fulfillment greatly outweighs financial concerns. Money should be a tool to help you achieve what you want, not an end in and of itself. What would she do if it wouldn't make a financial difference either way? You have enough buffer to experiment a little bit. 28/29 is way too young to feel like you are tied to a job you don't like. That said, has she fully explored other, less stressful, full-time options? Including applying for new jobs and going on interviews? If she's a manager, is that just the part that isn't right for her? And has she worked on ways to make the job she currently has less stressful before considering leaving? (Though the commute alone seems like a good reason to look for other work--or move!) Just wanted to say that it makes sense to a) do everything in her power to improve her current situation and b) understand what other options are realistic, before leaving this position. But if she has done that, you have my blessing to experiment a little bit :)