I would think about the types of things your wife likes to do and the types of people she enjoys spending time with. Think about how she spends her time in Florida and see if you can find ways for her to replicate that in Alabama -- Meetup groups, favorite restaurants, activities, places she shops (whether for fun or just for groceries, etc.), volunteer organizations, etc. Make it very specific to her, not just generic "things people like."
Maybe there's something you can do for her that would help ease the transition -- something fairly big that she's been wanting, whether it's a nicer car, a pet, a trip, etc. It would be nice if you could tie it in with the new location so that it seems more like a "hey, this comes conveniently along with our new home" rather than a bribe. Like, "We'll have a big backyard, so now we can get a dog," or "We'll live closer to X airport so we can get cheaper direct flights to see your family more often in Y."
But also yes, as others have mentioned, it would be helpful to find out whether this is just fear of change/the unknown versus a true hatred of the new place. Also, remind her that this doesn't necessarily have to be forever. I wouldn't turn it into a "We'll only stay 3 years" type of bargain, but like many changes in life, you can always change again if you decide that's best.