Yes, line 43 determines your tax bracket.
Your tax bracket is the % of tax attributed to the last $ of income you earned (It's also called the marginal rate). So if you are MFJ, based on the table below if you make $73,800 you are in the 15% bracket, and if you make $73,801 you are in the 25% bracket. This doesn't mean you pay 25% tax on the full amount, you likely would pay about 15.01% tax as a percent of your taxable income.
Most of the time your bracket is irrelevant, meaning earning $1 extra dollar to move into the next bracket will not hurt you. However, there are very, very important times when earning an extra dollar will hurt you very much. I used the 15% and 25% brackets for a reason as this drastically changes how capital gains and qualified dividends are taxed. Stay in the 15% bracket or lower if at all possible.
There are other intervals of income that are very important, but they aren't necessarily always tied to a bracket. These would be things like qualifying for the earned income credit, the savers credit, or AFA subsidies. They can be equally important.