The 160k is included in the 25x number, but you want to keep it invested, not pulled out and stuck not gaining returns. If it's not gaining returns, then it probably shouldn't be included in the 25x number.
Your 160k can be in taxable accounts, Roth contributions, or Roth (from tIRA) conversions. If you don't have any Roth contributions, you'll want to save it in your taxable account (and a little extra in case the market drops) so you can start your conversion ladder after retiring. So if you're close to retiring, and everything is saved in tIRA/401k, then you might want to stop contributing to those and put it in Roth or taxable. Sadly that will increase your tax rate for those years.